An analysis of complexes with large chimeric oligonucleotides shows that the basic tails of both proteins are attached flexibly, enabling them to bind rigid duplex DNA segments extending from the core in different directions. Our results indicate that the basic tails of DEAD-box proteins contribute to RNA-chaperone activity by binding nonspecifically to large RNA substrates and flexibly tethering the core for the unwinding of neighboring duplexes. Electric cells: You’ve heard of electric eels; how about electric bacteria? Researchers at Harvard, publishing in Science, were curious about the electrical properties of cells: Antenna tower construction crew: Cilia are complex organelles in the cell membrane that protrude into the intercellular medium, where they can sense the surroundings and perform other functions. Some are motile, like the cilia in the human respiratory tract that sweep foreign matter out with coordinated strokes. Cells build cilia using molecular ore-carts powered by kinesin-2 motors in a process called intra-flagellar transport (IFT), in which components are hoisted up into the cilium on trackways. Scientists suspected that additional factors regulate the construction of cilia. Now, two American molecular biologists publishing in Current Biology identified one such member of the construction crew, named KLP6, that moves independently of kinesin-2 and acts to reduce its velocity. Note: they found this in a particular kind of cell in a roundworm. Gatekeeper dance: Ion channels play many vital roles in our bodies. According to Science Daily, they are “essential for the regulation of important biological processes such as smooth muscle tone and neuronal excitability.” One such channel, named BK, performs large conductance of potassium ions in the presence of calcium ions. Scientists had thought that all ion channels followed a unified theory of activation. Researchers at the University of Texas at Austin have found that BK channels are not that simple. DNA orchestra: An article on PhysOrg about DNA translation begins, “Just like orchestra musicians waiting for their cue, RNA polymerase II molecules are poised at the start site of many developmentally controlled genes, waiting for the ‘Go!’-signal to read their part of the genomic symphony.” Researchers at Stowers Institute for Medical Research found that Super Elongation Complex (SEC), an assembly of some 10 transcription elongation factors, conveys the downbeat to the translation machine and “helps paused RNA polymerases to come online and start transcribing the gene ahead”. This quick-start device “reduces the number of steps required for productive transcription and allows cells to respond quickly to internal and external signals,” one of the researchers explained. Continuing the orchestra metaphor, the article said, “Transcriptional control by RNA polymerase II (Pol II) is a tightly orchestrated, multistep process that requires the concerted action of a large number of players to successfully transcribe the full length of genes.” Quality control inspectors: At the exit gate of the ribosome, where new proteins have just been assembled, molecular machines called chaperones stand at the ready to help them fold properly. A Stanford team studied two of these, the Signal Recognition Particle (SNP) and Nascent Chain associated Complex (NAC), which have the remarkable ability to work on a wide variety of proteins, helping them fold properly. Publishing in PLoS Biology, they said, The Science Daily article reported that a team at Howard Hughes Medical Institute produced the highest-resolution diagram ever of a sodium channel . In this excerpt, you can sense the fascination as the team looked down the channel for the first time in such detail: “We hope to gain insight into why they selectively let in sodium ions and nothing else,” the researchers said, “and how they respond to changes in the cell membrane voltage, how they open and close, and how they generate electrical signals.” The researchers have already spotted intriguing molecular movement, such as rolling motions of some functional parts of the sodium channel molecule and their connectors…. Bacterial membrane potential provides a major component of the driving force for oxidative phosphorylation, membrane transport, and flagellar motion. Yet this voltage is inaccessible to techniques of conventional electrophysiology, owing to the small size of bacteria and the presence of a cell wall. Little is known about the electrophysiology of bacteria at the level of single cells. So they checked. They observed E. coli bacteria producing electrical spikes at a rate of about one per second. The electrical charge is generated by ion channels in the membrane that create electrical gradients, working against the natural tendency of charges to cancel out. “Spiking was sensitive to chemical and physical perturbations and coincided with rapid efflux of a small-molecule fluorophore,” they said, “suggesting that bacterial efflux machinery may be electrically regulated.” In other words, they were not observing a stochastic effect, but a coordinated action of many ion channels that must organize their active transport mechanisms as a unit. They speculated that the spiking represents a stress response by the organism. Coordinated electrical response is known in higher organisms, like electric eels and humans, but “These simple estimates show that some of the tenets of neuronal electrophysiology may need rethinking in the context of bacteria.” Salt of the cell: If you enjoy having a healthy heart, brain and pancreas, thank your sodium channels. Like the BK potassium channels, sodium channels regulate a wide range of physiological activities. According to Science Daily, “Mutations in voltage-gated sodium channels underlie inherited forms of epilepsy, migraine headaches, heart rhythm disturbances, periodic paralysis, and some pain syndromes.” When the dentist numbs your gums, he is effectively blocking the local sodium channels from doing their job – sending pain messages to the brain. “The ribosome uses two active mechanisms to unwind messenger RNA during translation.” (Nature) “Crystal structures of [lambda] exonuclease in complex with DNA suggest an electrostatic ratchet mechanism for processivity.” (PNAS) “Mechanism of activation gating in the full-length KcsA K+ channel.” (PNAS) We further show interactions between the peptidyl-prolyl isomerase and Trp-Trp (WW) domains amplify the conduit response, and alter binding properties at the remote peptidyl-prolyl isomerase active site. These results suggest that specific input conformations can gate dynamic changes that support intraprotein communication. Such gating may help control the propagation of chemical signals by Pin1, and other modular signaling proteins. RNA linemen: We’ve all watched in the movies how rescue workers toss a line over a wall or pole so that they can climb up. Something like that happens with some machines that unwind RNA. In PNAS, a team of researchers described a helicase with the awful name “mitochondrial DEAD-box protein Mss116p” that acts as a general RNA chaperone. (They really should give these machines better names, like the Chuck Norris Hammerlock Clamp.) Not only does the machine clamp down on the RNA, it first latches onto it by means of a couple of tails that fasten onto the RNA, tethering the machine to its target: Transport vesicles are created when coat proteins assemble on a flat membrane, select cargo, and deform the membrane into a bud. The budded vesicle is then carried to its target organelle, where it docks by means of ‘tethers’ before undergoing membrane fusion. The vesicle coat was once thought to fall off as soon as budding was complete, but we now know the coat is important for binding the tethering factors that help the vesicle identify the correct organelle. Coat proteins and tethers must be removed before fusion can take place, but what triggers their disassembly has always been a mystery. A paper recently published in Nature now shows that, when one kind of transport vesicle docks with its target membrane, it encounters a kinase that breaks the bond between the coat proteins and the tethers, kick-starting the disassembly process. The problem she was considering was how the steps are coordinated: “how can a vesicle hang onto its coat long enough to reach its target, but shed it once it arrives?” Part of the answer appears to be in good management. Though not yet fully understood, “These results paint a picture of Sec23 as a master regulator of budding and fusion, participating in successive interactions that are regulated by phosphorylation.” She added, though, that “While this is an attractive model, it may not be the whole story.” Other protein machines interact in vital ways with Sec23. Her diagram illustrates several pieces locking together like Lego blocks, arriving and releasing at precise times in a process reminiscent of a space shuttle docking with the space station. This complexity undermines a claim on PhysOrg that “Endocytosis is simpler than expected.” There, researchers in the Netherlands found a simpler model for the way the coat molecules (clathrin) rearrange from flat to spherical during the process of vesicle formation. That’s only a minor aspect of a much larger multi-stage process involving many protein parts, and as this animation shows, the geodesic-style clathrin molecules are pretty clever little building blocks. Rubber baby copper pumper: In “Structural biology: A platform for copper pumps,” Nigel Robinson, writing in Nature, said, “Copper is vital to most cells, but too much is lethal. The structure of a protein that pumps copper ions out of the cytosol provides insight into both the pumping mechanism and how certain mutations in the protein cause disease.” His diagram shows a molecular machine called LpCopA, comprised of at least 5 protein domains, that safely pumps copper ions out of the cell. First, the ions have to be delivered to the pump with special chaperone molecules that know how to handle it safely. Inside the pump are three binding sites that deliver the ion to an L-shaped platform that gently holds the ion while undergoing conformational changes like a lever arm, ejecting the ion safely to the outside. Then the platform resets for the next round. Serious brain diseases can occur when these pumps are damaged by mutations. Our results provide new insights into SRP selectivity and reveal that NAC is a general cotranslational chaperone. We found surprising differential substrate specificity for the three subunits of NAC, which appear to recognize distinct features within nascent chains. Our results also revealed a partial overlap between the sets of nascent polypeptides that interact with NAC and SRP, respectively, and showed that NAC modulates SRP specificity and fidelity in vivo. These findings give us new insight into the dynamic interplay of chaperones acting on nascent chains. Stereo amplifier: Researchers at Notre Dame and Virginia Tech found that an important signaling enzyme named Pin1 is stereoselective. “Pin1 is a modular enzyme that accelerates the cis-trans isomerization of phosphorylated-Ser/Thr-Pro (pS/T-P) motifs found in numerous signaling proteins regulating cell growth and neuronal survival,” they wrote in PNAS, showing that conduit response differs if the enzyme binds on one channel instead of the other. Here’s the scoop for bio-geeks: A student’s view of a cell under a light microscope is misleading. It reveals only a tiny fraction of what is really going on. Within that package of life, invisible to the student’s gaze, complex machines work together in cellular factories. Signals pass back and forth in complex networks. Libraries of code are transcribed and translated into machine parts. Security guards open and close gates, and emergency response teams repair damage. Even a simple bacterial cell has the equivalent of a city council, library, fire department, police department, industrial center, transportation infrastructure, disposal and recycling center, civil defense system and much more. Here are just a few snippets from recent scientific papers that zoom in past the microscope lens to reveal wonders unimagined just a few decades ago. Halfway down in the activation channel, a certain amino acid residue M314 in one of the transmembrane proteins does a little dance, “rotating its side chain from a position in the closed state not exposed to the hydrophilic pore to one that is so exposed in the open state.” This conformational change is part of a larger validation process that ensures only the right ions make it through the selectivity filter. M314 “might not actually form the part of the activation gate that blocks ion passage, but that motions in the deep pore may be required for blocking ion passage elsewhere in the channel,” the article explained. So much for unified theories; “Importantly, they say, the study demonstrates that BK channel activation is not an open-and-shut case as previously suspected.” Readers may wish to investigate these additional papers published this month with intriguing titles: Dirigible doughboys: Sometimes cells build dirigible-like “transport vesicles” out of parts of the cell membrane, to float large cargo molecules to other organelles in the cell. This complex process involves many players, including proteins that coat the vesicle, and dockers that hold the dirigible when it comes in for landing; in the case of the cell, though, the dirigible’s coat fuses with the target organelle, so that the contents can enter safely through a membrane tunnel. Elizabeth Conibear [U of British Columbia] introduced some of the complexity of this process, called endocytosis, in Current Biology, saying, “When a coated transport vesicle docks with its target membrane, the coat proteins and docking machinery must be released before the membranes can fuse. A recent paper shows how this disassembly is triggered at precisely the right time.” The first paragraph of her review article described just a fraction of what goes on in these operations: Time does not permit referencing all these papers; click on the links to go to the abstracts. These represent part of the backlog of papers on cellular wonders. Each of them deserve a complete discussion. Many of them talk about how parts are “highly conserved [i.e., unevolved] from bacteria to humans,” and none of them attempt even a minimally-plausible account of how they might have emerged by chance. In these exciting days of opening the cellular black box, we should be standing in awe of the design (and the Designer), not ascribing the machinery inside to mindless, purposeless nothingness. The record speaks for itself. Researchers need Darwin like alcoholics need wine (Dar-wine). It turns them into WINOs, Wesearchers In Name Only (to be pronounced with a drunken drawl).(Visited 25 times, 1 visits today)FacebookTwitterPinterestSave分享0
Related Posts A first step towards creating a virtualized infrastructure often comes at a point when the customer starts experiencing performance issues. Servers start failing as application loads increase. When these issues start occurring, what are the first steps to take into consideration? How should a virtualized infrastructure be deployed? How are these pre-production environments developed? What are the most effective ways to deploy a simplified, reliable and optimized virtualization solution?Already we are seeing replies.But what do you think? Here’s a quick poll to help you think through what you want to say. A Web Developer’s New Best Friend is the AI Wai… Why Tech Companies Need Simpler Terms of Servic… Tags:#Analysis#cloud alex williams Top Reasons to Go With Managed WordPress Hosting The decisions that come with deciding how to use cloud computing reminds me of conversations that I am sure you’ve had.You look at a few options then you decide. But with the cloud, it’s about a few hundred features to consider. Our Road to the Cloud,” contest, sponsored by VMware and Intel, is looking at how companies get started in cloud computing. Write a comment and you will be a candidate to win a MacBook Air. We’ll be giving out a new MacBook Air every month.Here’s our first topic: 8 Best WordPress Hosting Solutions on the Market
Starting film school? Success is measured in more than just good grades. Check out these 10 tips to propel you to the top of the class.So you’ve taken the plunge. You’re starting film school and looking to pursue a career in the film and video industry. School is great for providing a solid basis of skills and build network connections, but to succeed post-graduation you’ll need to position yourself for success.Share your own thoughts and advice in the comments below!1. Start Shooting ImmediatelyWith the way college is structured, students usually have to take a full year’s worth of basic classes before they can actually start shooting film and video projects. Don’t fall into this trap. It’s better to get your bad films out as a freshman than to wait until you’re a senior with a terrible demo reel. Take the incentive to learn video/film production techniques on your own and you’ll be a step ahead.2. Volunteer to Help Out on ShootsOne way to develop your skills early on is to volunteer on projects other classmates are working on. There’s a lesson to learn on every shoot, even if that lesson is what not to do. See if there are any call boards in your school seeking crew members and connect with the classmates above you to see how you can help.3. Use the Barter System or RentYou don’t have to own a full studio worth of equipment to get proficient with different lighting and camera gear. Try bartering gear with other students – just be sure expectations are clear about responsibility and what would happen if the equipment were to be lost or broken. Another option is to rent equipment on a short term basis. Online rental houses like BorrowLenses, LensRentals and LensProToGo, provide high quality gear, nationwide shipping and affordable pricing.4. Become Friends with ActorsThey may be weird but they’ll work for pizza!Don’t make the mistake of asking your roommate to be in your student film. They will inevitably lose interest and your film will show it. Students pursuing an acting degree are often more than willing to be in your short films. It’s mutually beneficial – you get an actor, they get content for their demo reel. If you befriend an actor they may also open up connections to other talent.5. Don’t Get Bogged Down with TheoryA good portion of film school curriculum typically involves film theory and history. This academic approach to film works in a classroom setting, but isn’t applicable to most career goals of film students (unless, of course, you want to be a film professor). Many film schools are notorious for saying and not doing – which puts the initiative on you to get the production experience you need for post-graduate employment. Go shoot a film project, critique it, then shoot again. Being in school allows much freedom to explore and create – you may never have that same flexibility again.6. Realize You Need More than ‘Traditional’ SchoolHaving a college degree doesn’t guarantee you a job, especially in the film and video industry. You will need more than classes if you want to increase your chances of being employed when you graduate. You need applicable skills. Supplement your film school classwork with self-initiated study, either through online tutorials, in-person film/video workshops or on-set experiences (see #2).7. Specialize in SomethingYou’ve probably heard of the old phrase “jack of all trades, master of none.” While having a well rounded education is important, you should also try honing in on the areas that interest you most. Use the first year or two of film school to explore different production and post career paths. Experiment with shooting, editing, motion graphics, lighting, etc. Then, in the final year(s) of school try to get the most experience in the area you enjoyed most or excelled at. There’s a reason why Hollywood blockbusters require hundreds of people with very different, very specialized skill-sets.8. Learn Software EarlyHaving knowledge of post-production software is an extremely marketable skill that potential employers may consider. In fact, in many cases your application won’t even be considered if you don’t have proficiency in certain softwares.Often times, film schools will only have agreements with certain software providers, leaving other industry leading apps by the wayside. What you don’t have available at school, start learning on your own. Applications like Premiere Pro, Final Cut Pro, After Effects and DaVinci Resolve have widespread pro-level adoption, so it’s important to have some familiarity with each.9. Look for Gigs Outside of SchoolBeing in film doesn’t mean you have to work on only school related shoots. Look for shoots happening in your city or region. Companies are often more than willing to have “interns” work for free on their sets to gain experience. This can also be a great opportunity to connect with would-be potential employers.In larger metropolitan areas, check out popular online film/video job boards like Production Hub and Mandy.com, which often have open crew and post production positions listed.10. Use Your “Film Student” CardLeverage your position as a student. Don’t have money to rent a location? Let the owner know it’s for a school project and they will be much more likely to let you use it for free. Want to tour a studio? Most people are happy to show film students their studios. Find people in job roles that you may want to be in and email them. They are often very willing to answer questions regarding their work with students. Did you know you may even be able to write-off movie tickets because you’re a film student?‘Real life’ doesn’t begin when you graduate. You are in real life right now and the connections you make and work you produce will have the power to open up doors for the rest of your life.Any tips you’d like to add?Share in the comments below!
The international kite festival which attracts kite flyers from across the country and the world, began on Sunday in Ahmedabad. The international kite festival was inaugurated by Gujarat Chief Minister Vijay Rupani at Sabarmati Riverfront. The Governor O.P. Kohli was also present on the occasion. Kite enthusiasts from across India and also foreign countries are participating in the week-long festival that is being celebrated across cities in Gujarat. The state government-sponsored festival will culminate on January 14, which is celebrated as Uttarayan (Makar Sankranti). Around 150 delegates from 44 countries, including the UK, South Korea, New Zealand, China, Indonesia and Malaysia, have arrived here to participate in the event. Nearly 100 kite enthusiasts from 18 Indian states and hundreds of them from Ahmedabad also displayed their kite flying skills on the occasion. Chinese dragon kites and hundreds of kites tied to a single string hogged the limelight.“People from different countries have come here to fly kites. People from different states are also here. For around eight days, kite festivals will be celebrated in different cities in the state,” Rupani told reporters after opening the festival. He said the festival will give boost to the kite industry which employs around 3 lakh people. The state Congress, however, criticised the government over spending money in organising the kite festival. The Opposition party said the BJP government should instead provide relief to thousands of families associated with the kite industry by removing the 5% Goods and Services Tax (GST) imposed on the sector.
Mumbai: An investigation by the Aircraft Accident Investigation Board into the September 2013 helicopter crash in Tukawade, Thane, which killed all five on board, has revealed the accident was caused due to the pilot continuing the flight in very poor visibility conditions with insufficient ground clearance and hitting a hill due to spatial disorientation.The report, prepared in 2014 but made public only earlier this week, also mentions that non-functioning of the weather radar and non-familiarity of the pilot with the terrain along with lack of valid training on the specific type of helicopter, contributed to the accident.A three-member committee headed by R S Passi as chairman, Captain V M Satish Koikal and K Chandra Shekhar as members, investigated the crash. The helicopter, belonging to United Helicharters, was flying from Juhu to Aurangabad when it crashed on September 29, 2013. The deceased included Captain Surendra Bhadoria, co-pilot Alan Martin, additional pilot Anshu Matha, engineer Yatin Wakade and technician Dean D’Souza.The report said that from the cockpit voice recorder (CVR) transcript, it was clear the crew experienced poor visibility right from the beginning. “The pilot was not familiar with the terrain. The radar was unserviceable. The flight was continued in poor visibility and without adequate terrain clearance. The pilot tried to maintain ground contact, resulting in not having safe ground clearance,” the report read.The report also mentions the early take-off of the helicopter from Juhu had been necessitated by refusal of Aurangabad air traffic controllers to extend watch hours (duty timings) in the morning, thereby indirectly contributing to the accident. The committee also said it appeared that the pilot was under mental compulsion to complete the flight as there was not much flying by the company in the recent past and fulfilling the flying contract from Nagpur for 30 days was important from the commercial point of view.“No action was taken by the crew as per the existing instructions or procedures. The crew did not review the situation, nor did they abandon further flight due to the weather situation. As seen from the CVR readout they were encountering clouds almost from the beginning, the crew displayed undue eagerness to press ahead with the flight as per the flight plan in VFR despite poor visibility. At a distance of 85 kms from Mumbai, the pilot decided to fly on instruments,” the report revealed.
Don’t miss out on the latest news and information. The league had selected Charlotte to host the 2017 All-Star game, but later moved the game to New Orleans because of the state law restricting the rights of LGBT people. However, a compromise was struck in March to partially erase the impact of the House Bill 2 law limiting anti-discrimination protections for lesbians, gays, bisexual and transgender people.“While we understand the concerns of those who say the repeal of HB2 did not go far enough, we believe the recent legislation eliminates the most egregious aspects of the prior law,” NBA Commissioner Adam Silver said in a release. “Additionally, it allows us to work with the leadership of the Hornets organization to apply a set of equality principles to ensure that every All-Star event will proceed with open access and anti-discrimination policies.FEATURED STORIESSPORTSSEA Games: Biñan football stadium stands out in preparedness, completionSPORTSMalditas save PH from shutoutSPORTSPrivate companies step in to help SEA Games hosting“All venues, hotels and businesses we work with during All-Star will adhere to these policies as well.”Despite Silver’s intentions, the Equality NC and the Human Rights Campaign has concerns that no protections for non-discrimination policies for the LGBTQ community have been put in place by the Charlotte or the state. Cayetano dares Lacson, Drilon to take lie-detector test: Wala akong kinita sa SEA Games Sports Related Videospowered by AdSparcRead Next More than 5,000 measles deaths in DR Congo this year — WHO Adam Silver, commissioner of the National Basketball Association announces that the 2018 NBA All-Star game will be held in Los Angeles at Staples Center during a press conference at Staples Center on March 22, 2016 in Los Angeles, California. Sean M. Haffey/Getty Images/AFPCHARLOTTE, NORTH CAROLINA—The NBA All-Star game is headed back to Charlotte in 2019, a couple of years later than anticipated.The NBA announced that the All-Star weekend will be held Feb. 15-17 in Charlotte and the game will be played at the Spectrum Center, home of the Charlotte Hornets.ADVERTISEMENT Panelo suggests discounted SEA Games tickets for students PLAY LIST 01:35Panelo suggests discounted SEA Games tickets for students02:49Robredo: True leaders perform well despite having ‘uninspiring’ boss02:42PH underwater hockey team aims to make waves in SEA Games01:44Philippines marks anniversary of massacre with calls for justice01:19Fire erupts in Barangay Tatalon in Quezon City01:07Trump talks impeachment while meeting NCAA athletes LOOK: Vhong Navarro’s romantic posts spark speculations he’s marrying longtime GF South Korea to suspend 25% of coal plants to fight pollution ‘Coming Home For Christmas’ is the holiday movie you’ve been waiting for, here’s why LATEST STORIES SEA Games: PH beats Indonesia, enters gold medal round in polo MOST READ Going, going, gone at French Open Pagasa: Kammuri now a typhoon, may enter PAR by weekend “North Carolina’s discriminatory law prohibits the city of Charlotte from implementing non-discrimination protections for LGBTQ residents and visitors attending the All-Star Game. Nothing has changed that fact,” said HRC senior vice president for policy and political affairs JoDee Winterhof.The NBA is the latest sports entity to return events to North Carolina; the NCAA and the Atlantic Coast Conference also are bringing events back to the state after changes were made to the law.The now-repealed House Bill 2 required transgender people to use restrooms corresponding to the sex on their birth certificates in many public buildings. That’s been dropped, but LGBT advocates have denounced the replacement law because state officials took no action barring sexual identity and gender discrimination in workplaces, restaurants and hotels and instead prohibited local governments from acting on their own.Hornets owner and longtime NBA great Michael Jordan said in a release he is “thrilled” the game is coming back to Charlotte.“We want to thank Commissioner Silver for his leadership throughout this process and for the decision to bring NBA All-Star back to Buzz City,” Jordan said in the release. “All-Star Weekend is an international event that will provide a tremendous economic impact to our community while showcasing our city, our franchise and our passionate Hornets fan base to people around the world.”ADVERTISEMENT Jordan asked Silver to keep the city in mind for 2019 after the league moved the 2017 game – hopeful the HB2 law would eventually be repealed.Silver honored that request.Hornets COO and president Fred Whitfield represented the Hornets and Spectrum Center in doing whatever he could to help facilitate a resolution, spending time meeting with legislatures and other business leaders in North Carolina.“From the very beginning I was in engaged to see if we could not only save the 2019 All-Star game, but the NCAA (basketball) regionals and the ACC Tournament, as well as concerts and events in the building,” Whitfield said. “We are operators of the building and we felt like we had to get engaged to assist to get some resolution.”Even as talks to repeal HB2 stalled at times, the Hornets continued to move forward with the league’s request to upgrade the arena.The $41 million renovation – $33.5 million of which came from the City of Charlotte – is almost complete, and has included a new scoreboard, new floor and renovations to suites and hospitality areas, among other upgrades.Charlotte previously hosted the All-Star game in 1991 at the Charlotte Coliseum, which has since been demolished.Pete Guelli, the Hornets executive vice president and chief sales and marketing officer, estimates a $100 economic impact for the city, but said the reputational effect will be even bigger.“This city has changed significantly since the last time it hosted a game 28 years ago,” Guelli said, “and the opportunity to showcase that on an international stage is incalculable.” Lakers win 9th straight, hold off Pelicans View comments
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